About This Course
This lab is about molecular absorption spectroscopy. Spectroscopy is an essential tool for physical scientists and is included in many UG and PG curricula. Spectroscopy uses the absorption, emission, or scattering of electromagnetic radiation by atoms, molecules or their ions, is employed to study these species and many physical processes. Various kinds of molecular spectroscopic techniques, such as UV-Vis (absorption) spectroscopy, fluorescence (emission) spectrometry, CD/ORD spectroscopy, infrared spectroscopy, Raman spectroscopy, NMR spectroscopy, mass spectrometry etc. are used. All methods except mass spectrometry are based on electronic radiation. Depending on the wavelength of interaction, we get information about different parts of molecules, for example, UV-Vis and Fluorescence spectroscopies --- " electronic structure ", Infrared and Raman spectroscopies --- " vibrations and/or rotations " , CD/ORD spectroscopy --- " chirality ", etc. Absorption spectroscopy involves the transfer of energy from the radiation field to the atom or molecule. In other words, it involves the measurement of the wavelength and intensity of absorption of light by a sample.